Ez ladder protein western blot verification#

Sizing of proteins on SDS-PAGE gels and Western blots.Īpproximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25☌), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol. Monitoring of protein transfer onto membranes during Western blots. Monitoring of protein migration during SDS-PAGE. Ready-to-use: supplied in a loading buffer for direct loading on gelsĮasy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye Do not heats, dilute, and add reducing agent before loading.īroad range: 10-180 kDa (Tris-glycine-SDS running buffer) The ladder is supplied in gel loading buffer and is ready to use.

Ez ladder protein western blot verification#

The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with Tris-glycine-SDS running buffer. The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kilodalton (kDa).